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Immunohistochemical expression of NLRP3 in skeletal muscle in cows. (A) Aged cows (Group A), (B) Adult cows (Group B), (C) Young cows (Group C). HRP method with Mayer’s hematoxylin counterstain. (D) Immunoreactivity score for NLRP3 expression. There is a statistically significant negative association between age and the presence of NLRP3 immunolabeled muscle fibers. Each value is the mean ± SEM (* p < 0.05; *** p < 0.001).
