Figure 5.

Western blot analysis for NLRP3 inflammasome expression in bovine muscle during aging. Skeletal muscle tissue from young, adult and aged bovine was homogenized and the protein lysates were analyzed by Western blot using antibodies for NLPR3 and for GAPDH as control. The blots were detected by ECL and autoradiography. In skeletal muscle of young bovine there is an increase of immunoreactivity for NLPR3 inflammasome compared to adult and aged animals. Data are shown as mean ±SD and asterisks denote statistically differences (* p < 0.05; ** p < 0.01).