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. 2021 Mar 31;22(7):3645. doi: 10.3390/ijms22073645

Figure 1.

Figure 1

Morphological analyses of formalin-fixed and paraffin-embedded tissue sections from control murine EDL muscles, EDL muscles damaged by ex vivo forced eccentric contraction (EC) and EDL muscles damaged by EC in the presence of bone marrow-mesenchymal stromal cell (MSC) conditioned medium (EC + MSC-CM). (a–c) Representative light microscopic images of longitudinal muscle tissue sections stained with hematoxylin and eosin (H&E). Scale bars = 50 μm. (d–f) Representative superimposed confocal fluorescence and differential interference contrast (DIC, grey) images of muscle tissue sections showing nuclei stained with propidium iodide (PI, red). Scale bars = 25 μm. (g) Quantitative analysis of the percentage of damaged myofibers. Values are mean ± SEM. ** p < 0.01 vs. control, ## p < 0.01 vs. EC.