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. 2021 Mar 25;22(7):3392. doi: 10.3390/ijms22073392

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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SARS-CoV-2 infection of Calu-3 cells (A) TCID50 measurements of virus titres, (B) qRT-PCR measurements of intracellular viral RNA represented by 2-ΔΔCt normalised first to GAPDH and then to inoculum levels of SARS-CoV-2, set to 1, and (C) intracellular viral protein in Calu-3 cells infected with SARS-CoV-2 (MOI 0.1). (D) Immunofluorescence microscopy showing SARS-CoV-2 N protein staining (green) in Calu-3 cells infected with SARS-CoV-2 (MOI 0.1) at various timepoints. Cell nuclei were stained using DAPI (blue).