Figure 1.

Schematic representation of the assayed treatments including symptoms, ELISA and RT-PCR: (A) control healthy “GF305” peach rootstocks; (B) Plum pox virus (PPV)-infected “GF305” rootstocks showing severe sharka symptoms; (C) healthy “GF305” peach rootstocks grafted with “Garrigues”; (D) PPV-infected “GF305” rootstocks showing sharka symptoms grafted with “Garrigues”; (E) healthy “GF305” peach rootstocks grafted with “Garrigues”, which were later inoculated with PPV at the end of the first cycle of evaluation. In addition, “Garrigues” almond samples grafted onto healthy (F) and PPV-inoculated “GF305” (G) rootstocks were included in the study. PPV was evaluated by symptom observation in leaf, ELISA-test and RT-PCR analysis. Presence of virus using these methodologies was indicated with + and absence with -.