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. 2021 Mar 26;22(7):3435. doi: 10.3390/ijms22073435

Figure 3.

Figure 3

Genetic determinant of the subtype D psi phenotype. (A) Alignment of NL4-3 [10] and ELI strain psi sequences used in the experiment. The bases enclosed by red rectangles are discordant bases. The restriction enzymes applied for generating recombinants are shown. (B) First screening of the determinant. On the left, schematics of recombinant psi are shown. The parts from subtype B are white, and that from subtype D is gray. The data are the average of three independent experiments. Error bars represent SEM. (C) Second screening of the determinant. (D) Investigation of the base substitution mutants concerning five discordant bases (UAGGA/CGAAC) in NarI-BssHII fragment. (B–D) On the left, schematics of recombinant psi are shown. The parts from subtype B are white, and from subtype D are gray. The data are the average of three independent experiments. Error bars represent SEM. The red asterisks (*) on Figure 3A,D indicate the bases in our cloned ELI fragment, which are not identical with that of the ELI strain sequence registered in GenBank.