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. 2021 Mar 26;22(7):3435. doi: 10.3390/ijms22073435

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Nucleotide variations at the 226/227 dinucleotide pair and its flunking regions of HIV-1 psi segment. Shannon entropy scores representing variations at individual nucleotide positions were calculated using 815, 20, and 1556 sequences of HIV-1 subtype B, subtype D, and HIV-1 major subtypes, respectively [2,3] from different geographic regions in the world [34]. Nucleotide sequence of HIV-1 subtype B infectious molecular clone NL4-3 [10] is shown in horizontal axis along with corresponding Shannon entropy scores in y-axis. An entropy score of 0 indicates absolute conservation, whereas a score of 2.0 indicates complete randomness. Red arrow heads represent the dinucleotide at position 226 and 227.