Figure 7.

(I) Masson’s trichrome staining of ASC-based self-assembled scaffold in which the expression of collagen is detected in blue color. DF-based self-assembled scaffold was used as comparative control. Human native urethra was used as positive control. (II) Immunocytochemical staining of ASC-based self-assembled scaffold with anticollagen type I antibody in which the expression of collagen type I, is detected in red color (A,C). DF-based self-assembled scaffold was used as comparative control (B,D). 4’,6-diamidino-2-phenylindole (DAPI) was used as nuclear counterstain in blue color in all samples. The scale bar represents 100 μm. Results are from a representative of three independent experiments.