Table 1.
Gene expression profile of ASC-based self-assembled scaffold and gene regulation status vs. DF-based self-assembled scaffold.
| Position | Symbol | Gene Name | Fold Change | Fold Regulation |
|---|---|---|---|---|
| Genes Upregulated in ASC-Based Self-Assembled Scaffold vs. DF-Based Self-Assembled Scaffold | ||||
| A01 | ADAMTS1 | ADAM metallopeptidase with thrombospondin type 1 motif, 1 | 9.84 | 9.84 |
| A02 | ADAMTS13 | ADAM metallopeptidase with thrombospondin type 1 motif, 13 | 66.87 | 66.87 |
| A03 | ADAMTS8 | ADAM metallopeptidase with thrombospondin type 1 motif, 8 | 21.55 | 21.55 |
| A04 | CD44 | CD44 molecule (Indian blood group) | 13.88 | 13.88 |
| A05 | CDH1 | Cadherin 1, type 1, E-cadherin (epithelial) | 2.82 | 2.82 |
| A06 | CLEC3B | C-type lectin domain family 3, member B | 42.51 | 42.51 |
| A10 | COL14A1 | Collagen, type XIV, alpha 1 | 34.77 | 34.77 |
| A12 | COL16A1 | Collagen, type XVI, alpha 1 | 1.67 | 1.67 |
| B02 | COL4A2 | Collagen, type IV, alpha 2 | 5.51 | 5.51 |
| B03 | COL5A1 | Collagen, type V, alpha 1 | 2.19 | 2.19 |
| B06 | COL7A1 | Collagen, type VII, alpha 1 | 8.05 | 8.05 |
| B09 | CTNNA1 | Catenin (cadherin-associated protein), alpha 1, 102kDa | 8.05 | 8.05 |
| B10 | CTNNB1 | Catenin (cadherin-associated protein), beta 1, 88kDa | 2.36 | 2.36 |
| B11 | CTNND1 | Catenin (cadherin-associated protein), delta 1 | 2.18 | 2.18 |
| B12 | CTNND2 | Catenin (cadherin-associated protein), delta 2 (neural plakophilin-related arm-repeat protein) | 1323.36 | 1323.36 |
| C03 | HAS1 | Hyaluronan synthase 1 | 494.55 | 494.55 |
| C05 | ITGA1 | Integrin, alpha 1 | 1.52 | 1. 52 |
| C06 | ITGA2 | Integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor) | 14.38 | 14.38 |
| C07 | ITGA3 | Integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor) | 1.26 | 1.26 |
| C11 | ITGA7 | Integrin, alpha 7 | 37.44 | 37.44 |
| D05 | ITGB2 | Integrin, beta 2 (complement component 3 receptor 3 and 4 subunit) | 31.26 | 31.26 |
| D07 | ITGB4 | Integrin, beta 4 | 8.67 | 8.67 |
| D09 | ANOS1 | Kallmann syndrome 1 sequence | 72.84 | 72.84 |
| D10 | LAMA1 | Laminin, alpha 1 | 1.27 | 1.27 |
| D11 | LAMA2 | Laminin, alpha 2 | 1.93 | 1.93 |
| D12 | LAMA3 | Laminin, alpha 3 | 2.89 | 2.89 |
| E02 | LAMB3 | Laminin, beta 3 | 4.42 | 4.42 |
| E09 | MMP14 | Matrix metallopeptidase 14 (membrane-inserted) | 1.45 | 1.45 |
| E10 | MMP15 | Matrix metallopeptidase 15 (membrane-inserted) | 1.11 | 1.11 |
| F04 | MMP9 | Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) | 5.40 | 5.40 |
| F06 | PECAM1 | Platelet/endothelial cell adhesion molecule | 160.52 | 160.52 |
| F07 | SELE | Selectin E | 144.50 | 144.50 |
| F08 | SELL | Selectin L | 17.2 | 17.2 |
| F09 | SELP | Selectin P (granule membrane protein 140kDa, antigen CD62) | 7.2 | 7.2 |
| F12 | SPG7 | Spastic paraplegia 7 (pure and complicated autosomal recessive) | 238.85 | 238.85 |
| G01 | SPP1 | Secreted phosphoprotein 1 | 3.69 | 3.69 |
| G03 | THBS1 | Thrombospondin 1 | 1.22 | 1.22 |
| G04 | THBS2 | Thrombospondin 2 | 5.85 | 5.85 |
| G05 | THBS3 | Thrombospondin 3 | 82.90 | 82.90 |
| G07 | TIMP2 | TIMP metallopeptidase inhibitor 2 | 2.66 | 2.66 |
| G10 | VCAM1 | Vascular cell adhesion molecule 1 | 6.42 | 6.42 |
| G11 | VCAN | Versican | 1.95 | 1.95 |
| G12 | VTN | Vitronectin | 29.65 | 29.65 |
| Total | 43 | |||
| Genes Downregulated in ASC-Based Self-Assembled Scaffold vs. DF-Based Self-Assembled Scaffold | ||||
| A07 | CNTN1 | Contactin 1 | 0.93 | −1.06 |
| A08 | COL11A1 | Collagen, type XI, alpha 1 | 0.37 | −2.70 |
| A09 | COL12A1 | Collagen, type XII, alpha 1 | 0.09 | −10.35 |
| A11 | COL15A1 | Collagen, type XV, alpha 1 | 0.27 | −3.61 |
| B01 | COL1A1 | Collagen, type I, alpha 1 | 0.20 | −4.81 |
| B04 | COL6A1 | Collagen, type VI, alpha 1 | 0.52 | −1.90 |
| B05 | COL6A2 | Collagen, type VI, alpha 2 | 0.29 | −3.40 |
| B07 | COL8A1 | Collagen, type VIII, alpha 1 | 0.41 | −2.38 |
| B08 | CTGF | Connective tissue growth factor | 0.01 | −70.93 |
| C01 | ECM1 | Extracellular matrix protein 1 | 0.12 | −8.11 |
| C02 | FN1 | Fibronectin 1 | 0.05 | −17.56 |
| C04 | ICAM1 | Intercellular adhesion molecule 1 | 0.60 | −1.66 |
| C08 | ITGA4 | Integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor) | 0.44 | −2.26 |
| C09 | ITGA5 | Integrin, alpha 5 (fibronectin receptor, alpha polypeptide) | 0.10 | −9.71 |
| C10 | ITGA6 | Integrin, alpha 6 | 0.57 | −1.72 |
| C12 | ITGA8 | Integrin, alpha 8 | 0.45 | −2.19 |
| D01 | ITGAL | Integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide) | 0.37 | −2.64 |
| D02 | ITGAM | Integrin, alpha M (complement component 3 receptor 3 subunit) | 0.002 | −384.89 |
| D03 | ITGAV | Integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51) | 0.74 | −1.33 |
| D04 | ITGB1 | Integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) | 0.42 | −2.36 |
| D06 | ITGB3 | Integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61) | 0.08 | −12.46 |
| D08 | ITGB5 | Integrin, beta 5 | 0.22 | −4.51 |
| E01 | LAMB1 | Laminin, beta 1 | 0.04 | −20.11 |
| E03 | LAMC1 | Laminin, gamma 1 (formerly LAMB2) | 0.35 | −2.80 |
| E04 | MMP1 | Matrix metallopeptidase 1 (interstitial collagenase) | 0.36 | −2.75 |
| E05 | MMP10 | Matrix metallopeptidase 10 (stromelysin 2) | 0.36 | −2.71 |
| E06 | MMP11 | Matrix metallopeptidase 11 (stromelysin 3) | 0.03 | −27.09 |
| E07 | MMP12 | Matrix metallopeptidase 12 (macrophage elastase) | 0.06 | −15.41 |
| E08 | MMP13 | Matrix metallopeptidase 13 (collagenase 3) | 0.73 | −1.35 |
| E11 | MMP16 | Matrix metallopeptidase 16 (membrane-inserted) | 0.12 | −8.03 |
| E12 | MMP2 | Matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) | 0.45 | −2.20 |
| F01 | MMP3 | Matrix metallopeptidase 3 (stromelysin 1, progelatinase) | 0.16 | −5.99 |
| F02 | MMP7 | Matrix metallopeptidase 7 (matrilysin, uterine) | 0.003 | −273.10 |
| F03 | MMP8 | Matrix metallopeptidase 8 (neutrophil collagenase) | 0.12 | −8.03 |
| F05 | NCAM1 | Neural cell adhesion molecule 1 | 0.42 | −2.33 |
| F10 | SGCE | Sarcoglycan, epsilon | 0.31 | −3.16 |
| F11 | SPARC | Secreted protein, acidic, cysteine-rich (osteonectin) | 0.67 | −1.49 |
| G02 | TGFBI | Transforming growth factor, beta-induced, 68kDa | 0.05 | −18.80 |
| G06 | TIMP1 | TIMP metallopeptidase inhibitor 1 | 0.23 | −4.31 |
| G08 | TIMP3 | TIMP metallopeptidase inhibitor 3 | 0.34 | −2.86 |
| G09 | TNC | Tenascin C | 0.67 | −1.47 |
| Total | 41 | |||