Figure 5.

Antifungal activity of cell-free supernatant fractions of RHFS10. (A) 72-CFS was size-fractionated using 10, 30 kDa and, 50 kDa cutoff spin columns, and the obtained fractions were tested against M. phaseolina. The results obtained with fractions <10 (1), >10 (2), <50 (3) and >50 kDa (4) are reported. C+: Positive control, pentachloronitrobenzene; C−: negative control, toluene; RHFS10: 0.1 mL of fractionated 72-CFS. (B) Elution profile of 72-CFS by fractionation on Sephadex G-50 fine column chromatography. The antagonist activity of the three recovered peaks (1 mg/dot) is reported in the upper part of the panel. All data represent the average of three separate experiments.