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. 2021 Mar 24;22(7):3324. doi: 10.3390/ijms22073324

Figure 6.

Figure 6

Minimum inhibitory concentrations of purified fractions of 72-CFS on fungal growth. (A) Minimum inhibitory concentration of the antifungal compound present in pick 1 (Panel 1), pick 2 (Panel 2) and pick 3 (Panel 3) of purified fractions of 72-CFS using a 24-well plate assay. The commercial fungicide pentachloronitrobenzene (PCNB) (Panel 4) was used as a reference. The tested concentrations are indicated. Fungal plugs incubated with only PD broth (PD + M. phaseolina) and the PD alone (PD) were used as a control. The blue lines represent the MICs of the tested samples. (B) Graphical representation of the MIC assay. The dotted line indicates the starting size (mm) of M. phaseolina plug (4 × 4 mm) at the beginning of the experiment. The results were obtained after 5 days of incubation at 28 °C. Data are presented as means ± standard deviation (n = 3 replication for each different concentration). ANOVA statistical analysis is extremely significant indicated—**** p < 0.0001 and *** p < 0.001.