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. 2021 Apr 1;13(7):1632. doi: 10.3390/cancers13071632

Figure 2.

Figure 2

Induction of natural killer (NK) cell reactivity against CD133+ B-ALL cell lines. (A–D) The B-ALL cell lines SEM and RS4;11 were co-cultured with purified NK cells or PBMCs of healthy donors (effector to target (E:T) ratio of 2.5:1 or indicated E:T ratio) in the presence or absence of 293C3-SDIE and iso-SDIE (both 1 µg/mL) for 24 h (activation), 4 h (degranulation), or 2 h (Europium assay). To determine the NK cell activation and degranulation, the NK cells were identified as CD56+CD3− lymphocytes and stained with CD69 or CD107a with subsequent flow cytometric analysis. Target cell lysis of different B-ALL cell lines was analyzed by Europium assays. (A) Exemplary data of purified NK cells tested against the B-ALL cell line SEM. (B,C) Exemplary data from one PBMC donor (left panel) and pooled results of three PBMC donors tested with SEM (red) and RS4;11 (blue) (right panel) are shown (mean ± SEM). (D) Exemplary data from one PBMC donor (left panel) and pooled results of three PBMC donors tested with SEM (red) and RS4;11 (blue) at an E:T ratio of 80:1 (right panel) are shown (mean ± SEM). ns, not significant; * significant (p < 0.05).