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. 2021 Mar 25;26(7):1839. doi: 10.3390/molecules26071839

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) The effect of MVC on IL-9-producing CD8+ T cells was analyzed through flow cytometry in the spleen. (B,C) The expression levels of IL-9 mRNA (B) and protein (C) were analyzed by RT-PCR and western blot, respectively, in knee tissues from MVC-treated mice. (D) Representative dot plots of one mouse from each group. Normal control (NC) mice received 1% (v/v) DMSO in saline and MVC (50 mg/kg) intraperitoneally (ip) daily from days 21 to 35. CIA mice were treated with MVC (50 mg/kg) ip after the second immunization. The level of significance was set at * p < 0.05 compared with the CIA control group. Data are presented as mean ± SD (n = 6).