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. 2021 Mar 31;22(7):3632. doi: 10.3390/ijms22073632

Figure 2.

Figure 2

The pan-RAR antagonist AGN194310 ablates primary cells grown from pediatric primitive neuroectodermal tumor biopsies. (a) Metaphase fluorescence in situ hybridization (FISH) of a single cell stained with a probe (red) for the chromosome 1 centromere (six copies of chromosome 1 are visible). Scale bar = 100 µm. (b) Primary culture of cells from a pediatric primitive neuroectodermal tumor biopsy characterized by a neurosphere-like structure with differentiating cells. Scale bar = 100 µm. (c) Dose–response curves of cell viability for two primitive neuroectodermal tumors (i, PNET1) and (ii, PNET2) and an astrocytoma (iii, ACT1) following treatment with the pan-RAR antagonist AGN194310, the RARα-selective agonist AGN195183, or ATRA. Cells were plated into microtitre wells and cell viability determined by measuring the level of cellular ATP at day 5. Data are means ± SE of triplicate values from 3 (i), 2 (ii), and 4 (iii) independent experiments.