Table 5.

Long amplicon ddPCR cycling conditions for the TP53 splice mutation assays.

Assay	ddPCR Cycling Conditions
Intron 4 splice mutation	94 °C for 10 min, 50 cycles of 94 °C for 30 s, 64 °C for 1 min, 72 °C for 6 min, 98 °C for 10 min and then 12 °C hold
Intron 5 splice mutation	94 °C for 10 min, 50 cycles of 94 °C for 30 s, 62 °C for 30 s, 72 °C for 1 min, 98 °C for 10 min and then 12 °C hold
Intron 7 splice mutation	94 °C for 10 min, 50 cycles of 94 °C for 30 s, 62 °C for 1 min, 72 °C for 3 min, 98 °C for 10 min and then 12 °C hold