Table 6.
Description of primers and probes for TP53 t8, t8β and t8γ long amplicon ddPCR assay.
| Oligonucleotide | Location | Sequence (5′–3′) |
|---|---|---|
| Forward primer | Intron 2 | AGTGGATCCATTGGAAGGGCAGGC |
| Reverse primer 1 | Exon 10 | CTGGGCATCCTTGAGTTCC |
| α probe_HEX 1 | Exons 9/10 | 5HEX_CGGATCTGAAGGGTGAAATATTCTCCA_3IABkFQ |
| β probe_FAM_1 1 | Exon 9β | 56-FAM_ACTTTGCCTGATACAGATGCTACT_3IABkFQ |
| β probe_FAM_2 1 | Exon 9β | 56-FAM_TCTGTATCAGGCAAAGTCATAGAACCAT_3IABkFQ |
| γ probe_FAM 1 | Exons 9/9γ | 56-FAM_AGCATCTGAAGGGTGAAATATTCTCCA_3IABkFQ |
1 Reverse primer and probes the same as TP53 t1/t3/t4 and t5/t6/t7 multiplex long amplicon ddPCR assays [13].