Figure 3.
A2AR detection in living cells using a fluorescent ligand. HEK-293 cells permanently expressing A2AR and human peripheral blood mononuclear cells (PBMC) were incubated with 50 nM MRS7396 (MRS, red) in the absence or presence of 100 nM of ZM241385 (ZM). For live nuclear staining cells were incubated with 1µM Hoechst 33342 (blue). Scale bar: 20 μm.