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. 2021 Mar 30;13(7):1099. doi: 10.3390/polym13071099

Figure 4.

Figure 4

Characterization of cellular and bacterial response to bioprinted HB constructs in vitro. (A,B) Cellular growth (normalized to day 3) for C3H10T12 mouse cells (A) and human bone osteoblast (HBO) cells (B), measured by the noninvasive AlamarBlue assay for 17 days of in vitro culture. (C–F) Bacteriostatic effects of SPION in 2D culture (C,D) and in SPION-loaded HB constructs (E,F) were evaluated by culturing GFP+ S. aureus onto scaffolds for 24 h (C,E) and measuring fluorescence signals (D,F). * p < 0.05, ** p < 0.01 and **** p < 0.0001.