Table 3.
Diterpene glycosides organized by increasing retention times in an RP-C18 HPLC method.
| DG | RT (min) |
ΔRT (min) |
Oligosaccharide Positions | |
|---|---|---|---|---|
| C-13 | C-19 | |||
| Reb O | 2.92 | 0.16 | Glcβ(1-2)[Glcβ(1-3)]Glcβ1- | Glcβ(1-3) Rhaα(1-2)[Glcβ(1-3)]-Glcβ1- |
| Reb N | 3.08 | - | Glcβ(1-2)[Glcβ(1-3)]Glcβ1- | Rhaα(1-2) [Glcβ(1-3)]-Glcβ1- |
| Reb E | 3.08 | 0.18 | Glcβ1(1-2)Glcβ1- | Glcβ1(1-2)Glcβ1 - |
| Reb D | 3.26 | 0.23 | Glcβ(1-2)[Glcβ(1-3)]Glcβ1- | Glcβ1(1-2)Glcβ1 - |
| Reb J | 3.49 | 0.07 | Glcβ(1-2)[Glcβ(1-3)]Glcβ1- | Rhaα (1-2)Glcβ1- |
| Reb W | 3.56 | 0.20 | Xylβ(1-2)[Glcβ(1-3)]Glcβ1- | Rhaα(1-2) [Glcβ(1-3)]-Glcβ1- |
| Reb M | 3.76 | 0.04 | Glcβ(1-2)[Glcβ(1-3)]Glcβ1- | Glcβ(1-2)[Glcβ(1-3)]-Glcβ1- |
| Reb Y | 3.80 | 0.09 | Glcβ(1-2)Glcβ1- | Glcβ1(1-6)Glcβ1- |
| Reb V | 3.89 | 0.05 | Xylβ(1-2)[Glcβ(1-3)]Glcβ1- | Glcβ1(1-2)Glcβ1 - |
| Reb Z | 3.94 | 0.24 | Glcβ(1-2)[Glcβ(1-6)]-Glcβ1- | Glcβ1- |
| Reb U | 4.18 | 0.17 | Glcβ(1-2)[Glcβ(1-3)]Glcβ1- | Araα(1-6)-Glcβ1- |
| a | 4.35 | 0.50 | Glcβ(1-2)[Glcβ(1-3)]Glcβ1- | Glcβ1- |
| Reb T | 4.85 | 0.61 | Glcβ(1-2)[Glcβ(1-3)]Glcβ1- | Xylβ(1-2)[Glcβ(1-3)]-Glcβ1- |
| Reb H | 5.46 | 0.18 | Glcβ(1-3)Rhaα(1-2)[Glcβ(1-3)]Glcβ1- | Glcβ1- |
| Reb L | 5.64 | 0.71 | Glcβ(1-6)Glcβ(1-3)[Glcβ(1-3)]Glcβ1- | Glcβ1- |
| Reb I | 6.35 | 0.76 | Glcβ(1-2)[Glcβ(1-3)]Glcβ1- | Glcβ1(1-3)Glcβ1- |
| Reb A | 7.11 | 0.42 | Glcβ(1-2)[Glcβ(1-3)]Glcβ1- | Glcβ1- |
| Stev | 7.53 | 0.38 | Glcβ(1-2)Glcβ1- | Glcβ1- |
| Iso-Reb A | 7.91 | 0.65 | Glcβ(1-2)[Glcβ(1-3)]Glcβ1- | Glcβ1- |
| Iso-Stev | 8.56 | 0.52 | Glcβ(1-2)Glcβ1- | Glcβ1- |
| Reb F | 9.08 | 0.24 | Xylβ(1-2)[Glcβ(1-3)]Glcβ1- | Glcβ1- |
| Reb Z1 | 9.32 | 0.47 | Glcβ(1-2)[Glcβ(1-6)]Glcβ1- | H- |
| Reb C | 9.79 | 0.13 | Rhaα(1-2)[Glcβ(1-3)]Glcβ1- | Glcβ1- |
| Reb R | 9.92 | 0.34 | Glc(1-2)[Glcβ(1-3)]Xylβ1- | Glcβ1- |
| b | 10.26 | 0.16 | Xylβ(1-2)Glcβ1- | Glcβ1- |
| Dulc A | 10.42 | 0.08 | Rhaα(1-2)Glcβ1- | Glcβ1- |
| c | 10.50 | 0.69 | Glcβ(1-2)[Glcβ(1-3)]Glcβ1- | - |
| Reb G | 11.19 | 0.26 | Glcβ(1-3)Glcβ1- | Glcβ1- |
| d | 11.45 | 0.56 | 6-deoxyGlcβ(1-2)[Glcβ(1-3)]Glcβ1- | Glcβ1- |
| Reb L1 | 12.01 | 0.28 | Glcβ(1-3) Rhaα(1-2)[Glcβ(1-3)]Glcβ1- | H- |
| Reb H1 | 12.29 | 0.23 | Glcβ(1-6) Glcβ(1-3)[Glcβ(1-3)]Glcβ1- | H- |
| Rub | 12.52 | 1.80 | Glcβ1- | Glcβ1- |
| Reb B | 14.32 | 0.12 | Glcβ(1-2)[Glcβ(1-3)]Glcβ1- | H- |
| Iso-Reb B | 14.44 | 0.44 | Glcβ(1-2)[Glcβ(1-3)]Glcβ1- | H- |
| Stev-bio | 14.88 | 0.29 | Glcβ(1-2)Glcβ1- | H- |
| Iso-Stevbio | 15.17 | 0.15 | Glcβ(1-2)Glcβ1- | H- |
| Dulc B | 15.31 | 0.52 | Rhaα(1-2)[Glcβ(1-3)]Glcβ1- | H- |
| Reb F1 | 15.83 | 0.61 | Xylβ(1-2)[Glcβ(1-3)]Glcβ1- | H- |
| Dulc A1 | 16.45 | 0.12 | Rhaα(1-2)Glcβ1- | H- |
| Reb R1 | 16.57 | 0.70 | Glc(1-2)[Glcβ(1-3)]Xylβ1- | H- |
| e | 17.26 | 0.02 | Xylβ(1-2)Glcβ1- | H- |
| Reb G1 | 17.28 | 0.1 | Glcβ(1-3)Glcβ1- | H- |
| f | 17.36 | 2.19 | 6-deoxyGlcβ(1-2)[Glcβ(1-3)]Glcβ1- | H- |
| Stev-mono | 19.55 | Glcβ1- | H- | |