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. 2021 Mar 24;22(7):3293. doi: 10.3390/ijms22073293

Figure 2.

Figure 2

Antibodies (AB) against recombinant protein udTRF2 (the long linker region of TRF2). (a) Western blot of 1—induced bacteria lysate with commercial AB to TRF2 (Abcam); 2—induced bacteria lysate with anti-udTRF2 AB; 3—anti-udTRF2. AB reveals TRF2 in the human skin fibroblast lysate. Numbers on the left correspond to Mr of the marker. (b) ImmunoFISH staining of human skin fibroblasts: telomere probe—green; AB against TRF2 (ab13579, Abcam)—red. Scale bar = 10 µm. (c) ImmunoFISH staining of human skin fibroblasts: telomere probe—red; AB against udTRF2—green. (d) Immunostaining of human skin fibroblasts: AB against udTRF2—green; against TRF2 (ab13579, Abcam)—red. Scale bar = 7,5 µm. M—merged image. Representative confocal images, single z-slices, are shown. The results only indicate the coexistence of two molecules and do not provide any quantification.