Figure 5.

EV_MSC-T-HIF^c show immunoregulatory potential in a delayed-type hypersensitivity mouse model. BALB/c mice were sensitized by applying a 3% oxazolone solution on the abdominal skin at day −5 and the left ear was challenged with 1% oxazolone solution at day 0. Mice were injected with the following different treatments: PBS (black circle), EV_MSC-T^c (red triangle) or EV_MSC-T-HIF^c (blue square) 6 h after challenge. The non-challenged right ear was used as a no-inflammation control. (a) Ear swelling was recorded as an increase in thickness (in mm) at different time points. Data are presented as the mean ± SEM of seven mice in each group. Tukey’s multiple comparisons test was used for statistics. (b) Ear thickness measured at different time points. Data reflect the reduction in thickness caused by each treatment relative to the PBS control ear. Graph represents mean ± SD of seven mice in each group. Tukey’s multiple comparisons test was used for statistics. (c) Ear swelling measurement on histological sections. Bars represent the mean ± SD of thickness of six animals. Paired t-test was used for statistics. * p < 0.05, ** p < 0.01, *** p < 0.001. (d) Representative histological hematoxylin and eosin-stained sections of ears at the 72 h time point. Epithelial enlargement is noted in brackets. Scale bar: 15 μm.