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. 2021 Mar 25;22(7):3369. doi: 10.3390/ijms22073369

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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βThal⁺ RBC shape modifications and storage EV characteristics. (A) Percentage of non-reversible shape modifications in βThal⁺ and control RBC units throughout storage and representative micrographs captured by scanning electron microscopy (n = 8 per group; magnification: 1000×). (B–F) EV analysis (n = 5 per group, except procoagulant activity: n = 8 per group): procoagulant activity (B), total protein concentration (C), protein carbonylation (D), and protein tyrosine phosphorylation (E) levels in βThal⁺ and control EVs. (F) Representative immunoblots of individual proteins or protein modifications showing a trend for different expression levels in βThal⁺ vs. control EVs. Stomatin was used as the internal loading control. (*) p < 0.05 βThal⁺ vs. controls; F: fresh blood.