Table 1.

Cytotoxic activity (IC₅₀) of compounds 1–4 against MCF-7, HCT-116, HepG2 and WI-38 cell lines.

Compounds	* Cytotoxic Activity IC50 a (μM)
	MCF-7	HCT-116	HepG2	WI-38
1	26.8 ± 2.20	27.2 ± 1.11	28.8 ± 2.25	30.0 ± 1.20
2	21.6 ± 1.8	22.2 ± 1.5	25.5 ± 2.17	40.50 ± 2.25
3a	17.0 ± 1.80	15.2 ± 2.32	19.2 ± 1.50	38.23 ± 2.10
3b	18.50 ± 2.11	15.6 ± 3.15	19.4 ± 2.19	39.13 ± 1.30
3c	18.9 ± 2.00	16.3 ± 4.16	19.9 ± 2.06	37.42 ± 1.49
3d	19.2± 2.35	16.6 ± 1.27	20.2 ± 2.33	35.26 ± 2.42
3e	19.8 ± 2.70	16.2 ± 1.15	20.4 ± 2.30	40.01 ± 2.31
3f	10.7 ± 2.30	10.20 ± 1.18	10.6 ± 2.32	29.82 ± 1.51
3g	9.0 ± 2.40	8.5 ± 2.30	10.0 ± 2.46	30.01 ± 3.72
3h	10.2 ± 2.01	10.11 ± 2.45	10.4 ± 2.08	28.10 ± 4.22
4a	11.2 ± 4.31	12.3 ± 2.17	17.0 ± 4.30	38.03 ± 5.51
4b	11.6 ± 3.22	12.6 ± 1.05	18.0 ± 3.20	39.8 ± 4.51
4c	12.3 ± 4.08	13.2 ± 1.03	18.5 ± 4.38	29.5 ± 4.02
4d	12.6 ± 1.70	13.6 ± 1.09	18.7 ± 1.20	29.9 ± 3.63
4e	14.2 ± 0.74	13.7 ± 0.55	18.62 ± 0.94	20.24 ± 5.52
4f	8.01 ± 1.83	8.02 ± 0.26	8.9 ± 1.73	19.33 ± 5.87
4g	5.1 ± 1.14	5.02± 1.19	6.6 ± 1.40	16.32 ± 3.21
4h	6.6 ± 1.28	7.02 ± 0.46	7.5 ± 1.29	18.12 ± 2.06
5-FU	5.38 ± 0.24	7.88 ± 0.2	5.34 ± 0.4	5.70 ± 1.50
erlotinib	6.65 ± 0.82	7.49 ± 0.65	nd	22.50 ± 0.65

* Three independent experiments were performed for each concentration. ^a IC₅₀ values represent mean ± SD of three experiments.