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. 2021 Mar 31;22(7):3626. doi: 10.3390/ijms22073626

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Experimental design. A schematic diagram showing cell-type-specific culture stages, conditions, and timelines. hCD34+: primary human peripheral-blood-derived CD34+, HUDEP: cell lines HUDEP-1 and HUDEP-2, SFEM: Stem Span SFEM II, αMEM: Minimum Essential Medium Eagle with alpha modification, IMDM: Iscove’s Modified Dulbecco’s Medium, FBS: fetal bovine serum, SCF: stem cell factor, CC-100: StemSpan CC100, Dexa: dexamethasone, Dox: doxycycline, EPO: erythropoietin, HS: human serum, Holo-Tnf: holo-transferrin, Hep: heparin, 2-ME: 2-mercaptoethanol.