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. 2021 Mar 24;26(7):1831. doi: 10.3390/molecules26071831

Figure 4.

Figure 4

The effects of different doses of AG (1, 5 and 10 μM) with specific induction medium (IM) of each differentiation type on tri-lineage differentiation potentials of cells from SPFP-MSCs on day 21 compared with specific induction medium without AG as control. The scale bar (▬▬) in (A,B,D–F) represents 500 µm and 200 µm, respectively. (A) Osteogenic differentiation; Alizarin red S staining reflected that the calcium droplets. (B) Adipogenic differentiation; Oil Red O staining reflected that lipid droplets. (C) Chondrogenic differentiation; After a cell pellet was treated with chondrogenic medium and chondrogenic medium with AG. (D) Pellet cultures of SPFP-MSCs were sectioned and stained with Hematoxylin & Eosin reflected that morphology. (E) Pellet cultures of SPFP-MSCs were sectioned and stained with toluidine blue reflected that glycosaminoglycans. (F) Pellet cultures of SPFP-MSCs were sectioned and stained with alcian blue reflected that proteoglycans.