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. 2021 Mar 26;22(7):3401. doi: 10.3390/ijms22073401

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Co-immunoprecipitation of Na_v1.5 and TRPM4 in HEK-293 cells. (A) Left panel: Input signals confirming the expression of Na_v1.5 with or without FLAG tag and TRPM4. The alpha subunit of the Na/K pump was used as a loading control. Right panel: In the immunoprecipitated (IP) fraction, the signal was observed only in the presence of FLAG-Na_v1.5 and TRPM4. (B) Left panel: Input signals confirming the expression of Na_v1.5 with or without FLAG tag and TRPM5. Right panel: In the immunoprecipitated fraction, no signal was observed for TRPM5, even though the IP of FLAG-Na_v1.5 was successful.