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. 2021 Apr 1;22(7):3690. doi: 10.3390/ijms22073690

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Azidomyristoylation of transiently expressed hNaa40^L in HCT 116 cells. (Transiently hNaa40^L/S expressing) HCT 116 cells were metabolically labeled for 8 h with 50 or 100 µM azidomyristate or left untreated (-). Besides the well characterized NMT substrates PRKACA and c-Src, a specific enrichment of hNaa40^L could be observed in the eluates (lanes 5) obtained by streptavidin-aided affinity purification in case of the azidomyristate treated samples (middle and right panels). Tubulin-α (TUBA) served as negative control and * indicates a non-specific loading control. 1 to 5 correspond to equivalents of the input lysate, clicked lysate, clicked + precipitated lysate, supernatant after streptavidin pull-down and eluate, respectively.