Figure 3.

O-GlcNAcylation of TBP regulates metabolic gene expression. O-GlcNAcylation of TBP at the amino acid residue T114 impairs B-TFIID complex formation by disrupting BTAF1 binding. Disruption of TBP O-GlcNAcylation results in significant metabolic transcriptome reprogramming, leading to a profound alteration in lipid storage. Using O-GlcNAc as a nutrient sensor, cells can fine-tune the metabolic transcriptome. Dysregulation of O-GlcNAc signaling has catastrophic effects on metabolic gene expression.