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. 2021 Apr 1;22(7):3653. doi: 10.3390/ijms22073653

Figure 3.

Figure 3

miR-483-5p downregulates ERK1 at both the mRNA and protein level in HEK293 cells. (A) Scheme representing the predicted binding site for miR-483-5p in ERK1 and ERK2 3′-UTRs. (B) RT-qPCR analysis of ERK1 mRNA levels upon transfection with 10 nM and 50 nM of miR-483-5p mimic. GAPDH was used for normalization. (C) Immunoblot quantitative analysis of ERK1 protein levels upon transfection with miR-483-5p mimic at different concentrations. β-actin levels were used for normalization. (D) Representative immunoblot for ERK1/2 and pERK1/2 protein levels upon transfection with scramble and miR-483-5p mimic at 100 nM. (E,F) Immunoblot densitometry analysis of ERK1/2 and pERK1/2 protein levels upon transfection with scramble and miR-483-5p mimic at 100 nM. Data are shown as mean ± standard error from three independent experiments; Unpaired Student’s t-test was used for statistical analysis.