Figure 6.

U0126 inhibition of ERK1/2 phosphorylation decreases total and phosphorylated TAU levels in SK-N-MC cells. (A) Representative immunoblots of total and phosphorylated levels of TAU, ERK1 and ERK2 in SK-N-MC cells treated with vehicle (DMSO) or with 10 µM of U0126. β-actin was used as loading control. (B) Quantitative analysis of the immunoblot levels for TAU, ERK1 and ERK2. (C) Quantitative analysis of the immunoblot levels for pTAU, pERK1 and pERK2. Data are shown as mean ± standard error of the mean from three independent experiments; Unpaired Student’s t-test was used to compare groups and statistically significant differences shown with p values.