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. 2021 Apr 1;22(7):3653. doi: 10.3390/ijms22073653

Figure 7.

Figure 7

miR-483-5p-mediated downregulation of phosphorylated ERK1/2 is associated with a trend in decreased levels of phosphorylated TAU in SK-N-MC cells. (A) Representative immunoblots of total and phosphorylated levels of TAU, ERK1 and ERK2 in untransfected (mock) or transfected SK-N-MC cells with 100 nM miR-483-5p mimic. Cq values for miR-483-5p transfected cells are shown and in mock miR-483-5p was undetermined (Und). β-actin was used as loading control. (B) Quantitative analysis of the immunoblot levels for TAU, ERK1 and ERK2. (C) Quantitative analysis of the immunoblot levels for pTAU, pERK1 and pERK2. Data are shown as mean ± standard error of the mean from four biological replicates; Unpaired Student’s t-test was used to compare groups and we observed no statistically significant differences.