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. 2021 Apr 2;22(7):3704. doi: 10.3390/ijms22073704

Figure 2.

Figure 2

Western blot analysis of cas9 expression in C. acetobutylicum strains MGCΔcac1502 and CAS1. The strains were grown in Clostridium growth medium (CGM) liquid media with either glucose or xylose as the sole carbon source; the cell pellet was then subjected to an SDS-PAGE/Western blot using an antibody raised against Cas9 (Abcam). M: marker, 1: strain CAS1 grown on glucose, 2: strain CAS1 grown on xylose, 3: strain MGCΔcac1502 grown on glucose, 4: strain MGCΔcac1502 grown on xylose. The expected molecular weight of Cas9 is 160 kDa.