Table 5.
List of strains.
| Strain | Relevant Characteristics | Source or Reference |
|---|---|---|
| Bacterial strains | ||
| E. coli TOP10 | Invitrogen | |
| C. acetobutylicum | ||
| MGCΔcac1502 | ΔCA_C1502 | [5] |
| CAS1 | ΔCA_C1502, pyrE::pXyl_cas9 | This study |
| CAS2 | ΔCA_C1502, pyrE::pXyl_cas9, ΔCA_C2879 | This study |
| CAS2ΔldhA | ΔCA_C1502, pyrE::pxyl_cas9, ΔCA_C2879, ΔCA_C0267 | This study |
| CAS2Δptb-buk | ΔCA_C1502, pyrE::pxyl_cas9, ΔCA_C2879, ΔCA_C3075, ΔCA_C3076 | This study |
| CAS2ΔldhAΔptb-buk | ΔCA_C1502, pyrE::pxyl_cas9, ΔCA_C2879, ΔCA_C0267, ΔCA_C3075, ΔCA_C3076 | This study |