Figure 2.

The abscisic acid (ABA) content and distribution analyses in peanut during drought stress memory process using HPLC and immunofluorescence localization assays. The time points of D1-1 to R2-48 were sampled to observe the expression and distribution-changing trends. The untreated group was used as the control (D1-0). (a–c) The ABA content of the leaves, stems, and roots in peanuts during the drought stress memory process. The mean and SD were obtained from more than three biological replicates. (d–f) The ABA immunostaining of leaves, stems, and roots following the same treatment regime described for a. The control samples were treated with PBS; green fluorescence (arrows) indicates the presence of ABA; and no color indicates a lack of staining. Bars = 50 μm for leaf panels and 100 μm for the stem and root panels.