Table 2.
Suppressive effect of OA, CP, and Dexa on CD11b+Gr-1+(high) neutrophils and CD11b+SiglecF+ eosinophils in the BALF and lung of OVA-induced asthmatic model mice.
| Cell Phenotypes in BALF and Lung |
OVA-Induced Airway Inflammation Murine Model (Absolute No.) | |||||||
|---|---|---|---|---|---|---|---|---|
| Normal | Control | Dexa. 3 mg/kg |
OA 10 mg/kg |
OA 20 mg/kg |
CP 100 mg/kg |
CP 200 mg/kg |
||
| CD11b+Gr-1+ (×105 cells) |
BALF | 0.96 ± 0.13 | 1.72 ± 0.70 | 0.39 ± 0.12 * | 0.27 ± 0.12 * | 0.48 ± 0.08 * | 0.62 ± 0.13 | 0.21 ± 0.04 * |
| CD11b+SiglecF+(×105 cells) | 1.26 ± 0.20 | 42.8 ± 3.11 ### | 7.52 ± 0.73 *** | 22.36 ± 3.80 *** | 12.3 ± 2.21 ** | 26.7 ± 3.63 ** | 9.34 ± 1.02 *** | |
| CD11b+Gr-1+ (×105 cells) |
Lung | 5.11 ± 0.36 | 19.31 ± 1.10 ### | 5.30 ± 0.60 *** | 7.88 ± 0.70 *** | 5.87 ± 0.19 *** | 8.26 ± 0.69 *** | 5.50 ± 0.47 *** |
| CD11b+SiglecF+(×105 cells) | 6.65 ± 0.30 | 39.0 ± 2.90 ### | 12.61 ± 1.28 *** | 22.49 ± 1.40 *** | 14.99 ± 1.24 *** | 16.50 ± 1.18 *** | 12.53 ± 1.42 *** | |
Samples were measured on a flow cytometer and analyzed by two-color flow cytometry using a FACSCalibur device and CellQuest software (BD Biosciences, Mountain View, CA, USA). Absolute cell number of CD11b+Gr-1+(high) neutrophils and CD11b+/SiglecF+ eosinophils present in the BALF and lung. The statistical significance of differences between control and treatment groups was assessed by ANOVA and Dunnett’s multiple comparison test. Each point represents the mean ± SEM values for 6 mice. * p < 0.05, ** p < 0.01, and *** p < 0.00, for the OVA control group versus the experimental group comparisons. # p < 0.05, ## p < 0.01, and ### p < 0.001 for the OVA control group versus the normal group comparison. Normal, Normal BALB/c mice; Control, OVA inhalation plus vehicle; Dexa., OVA inhalation plus dexamethasone, 3 mg/kg; OA, OVA inhalation plus OA, 10 or 20 mg/kg; CP, OVA inhalation plus CP (100 or 200 mg/kg).