Figure 3.

Effect of free and nano-encapsulated MTM in CSC-enriched 3D sarcoma cell cultures. (A) Cells were plated at low density in tumorsphere medium and left to form tumorspheres for 10 days before treating them for 72 h with increasing concentrations of MTM. Treatments with an amount of empty nanoparticles corresponding to 10 µM (Ce) were also included. Scale bars = 250 μm. (B,C) Quantification of the spheres (represented as % of control) remaining after the treatment with MTM-loaded nanoparticles; (B) or with empty nanoparticles (C). Error bars represent the standard deviation of three independent experiments.