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Inhibition of SP1 signaling by free and nano-encapsulated MTM. Western blotting analyses of SP1 and several SP1 downstream targets in T-5H-FC#1 cells treated with the indicated concentrations of the different MTM formulations for 24 h. A treatment with empty nanoparticles (Ce) at a concentration equivalent to the higher encapsulated MTM dosage was also included. The expression of β-actin was used as the loading control.
