Figure 2.
Changes in anaerobic glycolysis caused by lactate calcium salt (CaLac) influx into colorectal cancer (CRC) cells CaLac acts as a substrate for the reactivation of lactate dehydrogenase B (LDHB) and induced the conversion of lactate into pyruvate. (A) Confirmation of LDHB activity following 2.5 mM CaLac treatment in CRC cells. 2 × 106 cells were analyzed respectively. (B) Confocal micrographs of LDHB reactivation in CRC cells following 2.5 mM CaLac treatment. Scale bars, 100 μm. (C) Quantitative analysis for GFP activity using Image J. (D,E) Representative pictures by IVIS for FITC-tagged LDHB expression following 2.5 mM CaLac treatment in CRC cells. And, quantitative analysis of LDHB expression. (F) Intracellular pyruvate levels following 2.5 mM CaLac treatment; 2 × 106 cells were analyzed respectively. ** p < 0.001 vs. Untreated. Results are represented as mean ± standard deviation.