Skip to main content
. 2021 Mar 25;22(7):3388. doi: 10.3390/ijms22073388

Figure 7.

Figure 7

Western blotting analysis. (A) Representative immunoblots through western blotting analysis for TG2 isoform (tissue transglutaminase long, TG2-L; tissue transglutaminase short, TG2-S) expression levels in total cellular lysate from OECs in different conditions: control, DMSO, 100 µM Indicaxanthin (INDI), 10 μM Aβ(1-42) or Aβ(25-35) or Aβ(35-25) both in the absence and presence of 100 µM INDI for 24 h; (B) Densitometric analysis of TG2-L expression levels performed after normalization with β-tubulin. (C) Densitometric analysis of TG2-S expression levels performed after normalization with β-tubulin. The results are expressed as the mean ± S.D. of the values of five separate experiments performed in triplicate. * p < 0.05 significant differences vs. controls; $ p < 0.05 significant differences of Aβ(1-42) + INDI or Aβ(25-35) + INDI or Aβ(35-25) + INDI vs. INDI; § p < 0.05 significant differences of Aβ(1-42) + INDI vs. Aβ(1-42), Aβ(25-35 ) + INDI vs. Aβ(25-35) or Aβ(35-25) + INDI vs. Aβ(35-25); & p < 0.05 significant differences of Aβ(1-42) vs. Aβ(25-35) or vs. Aβ(35-25) and of Aβ(1-42) vs. Aβ(25-35) + INDI or vs. Aβ(35-25) + INDI; # p < 0.05 significant differences of Aβ(25-35) vs. Aβ(1-42) or vs. Aβ(35-25) and of Aβ(25-35) + INDI vs. Aβ(1-42) + INDI or vs. Aβ(35-25) + INDI.