Figure 4.

Identification of AML patient subsets based on their constitutive protein release during in vitro culture; an unsupervised hierarchical cluster analysis including 40 patients and based on 1770 proteins detected for at least 50% of the patients. The cells were cultured for 48 h in serum-free medium before supernatants were harvested and the proteomic analyses performed. The patients clustered into two main clusters (yellow/upper and brown/lower; see the column on the right side of the clustering); each of these two main clusters had two subclusters (upper yellow/dark yellow and lower brown/dark brown, respectively). As can be seen from the upper part of the figure, the proteins clustered into five main clusters each including 392 proteins (Cluster 1, purple), 460 proteins (Cluster 2, green), 337 proteins (Cluster 3, yellow), 417 proteins (Cluster 4, orange) and 164 proteins (Cluster 5, blue), respectively. Patient characteristics are indicated to the right in the figure, and blank fields indicate information not determined. Black color in the cluster analysis indicates that the protein was not detected.