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Nucleosome cluster formation after irradiation of GFP-H2B-labeled HeLa cell nuclei with a dose of 3.5 Gy (X-rays) at energies of 6 MeV (LINAC) or 50 keV (IORT). Comparison to random H2B distribution is also provided. In the non-irradiated control cells (shown at 48 h of cultivation), 13% of signals followed the cluster criterion. See text for further details.
