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Hard protein corona quantification by micro-BCA assay. All samples were treated identically and subject to three cycles of washing with PBS. Protein corona formation on the surface of lyophilized dBSA-UCNP-NOBF4 was calculated after eliminating the dBSA background. The results were analyzed with an unpaired t-test (n = 5; *: p < 0.05, ***: p < 0.001) using GraphPad Prism 9.
