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Enzymatic hydrolysis of XGO1 after 20 h by using LacS and XylS.
| Enzymes | Galactose (µg) | Glucose (µg) | Xylose (µg) |
|---|---|---|---|
| TFA hydrolysis 1 | 365 ± 16 | 835 ± 26 | 803 ± 9 |
| LacS 2 + XylS | 12.3 ± 1.1 | 158.3 ± 1.3 | 149.7 ± 1 |
| LacS 3 + XylS | 41.3 ± 2.9 | 348.9 ± 9.3 | 299.9 ± 9.6 |
| LacS1 | ND | ND | ND |
| LacS2 | ND | ND | ND |
| XylS | ND | ND | 89.1 ± 9 |
Trifluoracetic acid (TFA) hydrolysis 1: 2 h at 100 °C in 2 M of TFA; LacS 2: 2.2 units; LacS 3: 18 units.
