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. 2021 Mar 24;22(7):3325. doi: 10.3390/ijms22073325

Table 2.

Enzymatic hydrolysis of XGO2 after 20 h by using LacS, XylS and SsαFuc.

Enzymes Fucose (µg) Galactose (µg) Glucose (µg) Xylose (µg)
TFA hydrolysis 1 83 ± 2 220 ± 18 346 ± 25 306 ± 11
LacS + XylS + SsαFuc 75.5 ± 5 6.6 ± 1 34.7 ± 5.8 115 ± 13
LacS + XylS ND 3.15 ± 0.1 35 ± 0.5 118 ± 3.9
XylS +SsαFuc 80.1 ± 14 ND ND 72.8 ± 9
LacS + SsαFuc 79.5 ± 9 4.7 ± 0.3 24.2 ± 4 ND
SsαFuc 73.3 ± 11 ND ND ND
LacS ND 1.5 13.6 ND
XylS ND ND ND 73.5 ± 11

TFA hydrolysis 1: 2 h at 100 °C in 2 M of TFA.