Figure 3.

Developmental and morphological defects in nej mutant embryos are partially ameliorated by VPA or NaB treatment. (a) wingless (wg) staining of nej mutant embryos treated as indicated. Representative projections of confocal imaging from stages 8 to 12 are shown. The defects detected in nej mutant embryos with or without treatment include loss or uneven wg staining (Drilled, arrow), twisting (arrowhead), presence of bottlenecks (double-arrowheads), or cracks (dashed-arrow). (b) Quantification of embryo survival and of the above phenotypes in nej mutant embryos at stages 8 to 12. VPA or NaB treatments partially rescue embryo development, allowing nej mutant embryos to survive beyond stage 8 although with aberrant phenotypes, with significant embryo survival at stage 10 compared to untreated embryos (p > 0.01). Student’s t-test was used as statistical method for comparing nej groups survival, with p < 0.05 considered significant.