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. 2021 Mar 29;22(7):3525. doi: 10.3390/ijms22073525

Figure 7.

Figure 7

Photoexcitation of OxDHE liposomes with visible light in the presence of 5,5-dimethyl-1-pyrroline N-oxide (DMPO) as the spin trap DMPO results in superoxide dismutase (SOD)- and catalase-dependent accumulation of DMPO-●OH adducts. (A,B): Representative electron spin resonance (ESR) spectra acquired from samples of liposomal suspensions made from oxidised (OxDHE, A) or freshly opened 16:0-22:6PC (DHE, B) exposed in the presence of 0.1 DMPO to 27 mW/cm2 visible light inside the resonant cavity of ESR spectrometer. The spectra were acquired at indicated irradiation times in the absence and presence of 0.1 mg/mL superoxide dismutase (SOD), catalase, catalase inactivated by irradiation with UV light, or bovine serum albumin (BSA). (C) a simulated spectrum of DMPO adduct with the hydroxyl radical (DMPO-●OH) allowing for identification of the adduct in the experimental spectra. (D) representative kinetics of DMPO-●OH adduct accumulation in the suspension of liposomes made from DHE or OxDHE in the dark (as indicated in the legend) or during irradiation with light (blue and cyan symbols and lines) in the absence and presence of SOD or catalase (Cat).