Table 3.
Candidate genes identified in the targeted QTL regions are strongly suggested to be responsible for the tested root and shoot traits in the nine pairs of wheat near-isogenic lines (NILs) used in this study.
| NIL Pairs | Traits with Significant Differences between Isolines | SNP | Candidate Gene | Gene Function | Marker–Gene Distance |
|---|---|---|---|---|---|
| 1–4 | RSR, RSA1, RSA2, RDCL1, RDCL2, SRL, SH, and NLP | Excalibur_c869_2091 | TraesCS4B01G114500 | Phox-associated domain, sorting nexin isoform 3 | 271.82 kb away |
| TraesCS4B01G114800 | bHLH-MYC and R2R3-MYB transcription factors N-terminal | 132.78 kb away | |||
| 5–7 | RL, RM, RSR, RSA2, RDCL1, SRL, and NLP | BobWhite_c22126_94 | TraesCS4A02G163700 | Inositol-1-monophosphatase family protein | 1064.77 kb away |
| BobWhite_c27287_232 | TraesCS4A02G330900 | LRR-RLK | 7.78 kb away | ||
| TraesCS4A02G331000 | F-box family protein | 17.30 kb away | |||
| BS00098868_51 | TraesCS4A02G215800 | 202.63 kb away | |||
| TraesCS4A02G215700 | 214.39 kb away | ||||
| BS00094406_51 | TraesCS4A02G185300 | UDP-glucoronosyl and/or UDP-glucosyl transferase | 962.14 kb away | ||
| CAP12_c3789_390 | TraesCS4A02G442700 | 11.58 kb away | |||
| RAC875_c51781_771 | 10.29 kb away | ||||
| Tdurum_contig12899_342 | TraesCS4A02G323500 | Protein kinase; serine–threonine/tyrosine- protein kinase | 5.86 kb away | ||
| TraesCS4A02G323400 | 10.11 kb away | ||||
| Jagger_c9935_144 | TraesCS4A02G000300 | 90.30 kb away | |||
| Excalibur_c94546_61 | TraesCS7A02G101000 | GH3 family, GH3 auxin-responsive promoter | 31.67 kb away | ||
| 9–10 | RL, Rdia, RDCL1, RDCL2, and NNR | CAP8_c3496_118 | TraesCS7A02G431600 | F-box domain | Overlapped in UTR |
| TraesCS7A02G431500 | Protein kinase; serine–threonine/tyrosine- protein kinase | 11.24 kb away |
RL = total root length, RM = root dry mass, RSR = root/shoot ratio, RSA1 = root surface area (>0.25 mm diameter class), RSA2 = root surface area (<0.25 mm diameter class), RDia = root diameter, RDCL1 = root diameter class length (<0.25 mm), RDCL2 = root diameter class length (>0.25 mm), SRL = specific root length, NNR = nodal root number per plant, SH = shoot height, NLP = leaf number per plant; SNP = single nucleotide polymorphism