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. 2021 Mar 30;13(7):1584. doi: 10.3390/cancers13071584

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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A streamlined approach to assess tumor suppressor gene candidates and oncogene function in human MPNST cell lines reveals novel MPNST pathways. (A) Diagram depicting summary of candidate selection from sleeping beauty (SB) screen to CRISPR/Cas9 forward genetic screening. (B) Diagram depicting screening method. First, gRNAs were designed to target genes selected in (A) and then stable cell lines were created and assessed for transformation upon genetic knockout. (C,D) show a summary of scores and anchorage-independent growth for predicted and known tumor suppressor gene candidates (1.5× magnification).