Figure 3.

Rho is a MPNST pathway and its negative regulator GDI2 is a novel Schwann cell tumor suppressor gene. (A) Western blot showing knockout of GDI2 in an immortalized Schwann cell line and levels in S462 MPNST cells. (B) Loss of GDI2 results in transformation as seen by increased anchorage-independent growth cell migration and tumor formation in NRGs (1.5× magnification). (C) Loss of GDI2 results in FAK activation and treatment with defactinib, a Rho inhibitor results in decreased anchorage-independent growth (2× magnification). Digital Western blot (Wess by Protein Simple) shows decreased FAK phosphorylation in GDI2-deficient cells upon treatment with defactinib. (D) Loss of GDI2 results in increased YAP levels and nuclear localization.