Intrathecal administration of BK evoked mechanical hypersensitivity. Administration of BK (21.2 μg, i.t., n = 6) evoked transient mechanical allodynia using von Frey filaments with bending forces 19 mN, 35 mN, and 59 mN (A), and hyperalgesia using filaments with bending forces 80 mN, 140 mN, and 290 mN (B). Data represent the mean ± S.E.M. of 6 (BK) and 6 (vehicle) independent experiments. Statistical analysis: Mann-Whitney rank test; * p < 0.05, ** p < 0.01, *** p < 0.001 left hindpaw, BK versus vehicle treatment; #
p < 0.05, ##
p < 0.01, ###
p < 0.001 right hindpaw, BK versus vehicle treatment. The time of the i.t. administration is marked by arrows.